Amplicon Sequencing

Amplicon Sequencing

As a long-established bio-sequencing company, CD Genomics provides researchers with a precise, high throughput amplicon sequencing service. The amplicon sequencing, we provide is a highly targeted approach that helps researchers analyze genetic variants, hotspot mutations, copy number variations, gene fusions, insertional deletions, and single nucleotide polymorphisms (SNPs) in specific genomic regions. It can also be used to identify and compare bacteria in a given sample, such as 16S rRNA sequencing.

What Is Amplicon Sequencing?

Amplicons are amplified fragments of a gene obtained by PCR amplification. In amplicon sequencing, next-generation sequencing technology is used to obtain the sequence of a PCR fragment targeting a specific genomic region and perform deep sequencing of the fragment. Through PCR amplification, pooling, and very high coverage of specific regions of interest, amplicon-seq can detect very low levels and frequencies of variants. At the same time, the method allows for multiplexing of samples, allowing the sequence of hundreds of PCR fragments to be determined simultaneously.

Customers Need to Provide

Samples

• For cell samples, the total amount should be greater than 2 × 10⁵.
• For DNA samples, the total amount is 15-20 µg.
• Both fresh/frozen animal and plant tissues can be shipped.

Please note that all samples should be shipped on an adequate amount of dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Bacterial macrogenomics studies.
• Detection of gene variants.
• Phase-change analysis.
• Identification and typing of microorganisms.
• Identification of genome editing.
• Identification of somatic mutations in complex samples.

Our Advantages

• Low sequencing costs and fast turnaround times.
• Ability to provide flexibility in experimental design for all species.
• Highly targeted sequencing even in regions with high GC content.
• High genomic coverage, supporting multiplexing of hundreds to thousands of amplicons per reaction.

Our Workflow

CD Genomics offers specialized amplicon sequencing services for researchers in a variety of research areas such as variant discovery or DNA methylation analysis. If you are interested in this, please feel free to contact us.

For research use only, not for any clinical use.