ChIP-Seq

ChIP-Seq

CD Genomics has a long-term focus on next-generation DNA sequencing technology, and with our professional sequencing platform and technical team, we can provide a high-quality and efficient ChIP-seq service to a wide range of research teams. We can effectively solve all kinds of problems in sequencing and provide strong technical support for your research.

What Is Chip-Seq?

Chromatin immunoprecipitation (ChIP) is a method that specifically enriches DNA fragments bound to target proteins in living cells to obtain information on protein-DNA interactions. The target protein was screened by ChIP, and we obtained the binding position of the protein and DNA by next-generation sequencing. ChIP-seq on the Illumina sequencing system supports almost any protein that can be immunoprecipitated by chromatin.

Customers Need to Provide

Samples

• We recommend sending ChIP-enriched DNA at no less than 10-50 ng per sample. samples should be submitted in 1.5-1.7 mL microtubes or 2 mL screw-capped test tubes. Since DNA concentration measurements with nanodropper can be very inaccurate at low concentrations (<20 ng/µL), report the ratio of A260/280 to A260/230. We recommend that elution of DNA is best performed in PCR grade H₂O, Tris, or low concentration EDTA TE (10 mM Tris, 0.1 mM EDTA).
• For cell samples, the number of cells needs to be greater than 2 × 10⁶.
• For fresh/frozen tissue samples, >500 mg for animal tissues and >1 g for plant tissues; for cross-linked tissue samples, >500 mg for animal tissues and >1 g for plant tissues.

Please note that all samples need to be stored in a -80℃ refrigerator and transported on dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Discover transcription factor binding sites.
• Uncovering the causes of epigenetic inheritance.
• Directly compare gene regulation in cells in normal and disease states.
• Identify genes regulated by known transcription factors and co-regulators.
• Analyze the effects of drug actions and other stimuli on regulatory pathways.

Our Advantages

• Well-established automated sequencing platform to provide high-quality ChIP-seq data
• Advanced cell nuclei isolation and purification technology to realize the experimental needs of multiple species.
• Professional technical team to provide personalized solutions to problems arising during ChIP-seq.

Our Workflow

CD Genomics is a biotechnology company specializing in ChIP-seq. Histone modification-mediated regulation of gene expression plays an important role in almost all biological processes, and we can provide technical support for your ChIP-seq studies. If you need, please feel free to contact us for technical support.

For research use only, not for any clinical use.