BCR Sequencing

BCR Sequencing

B-cell receptor (BCR) sequencing is used in the study of immune-related diseases to help researchers deepen their understanding of antibodies and the immune system. CD Genomics is a one-stop biotech company that provides sample extraction, library building, sequencing, bioinformatics analysis, and data mining services, and has the experience to solve any challenges you may encounter during your research.

What Is BCR Sequencing?

B-cell receptors are transmembrane proteins on the surface of B cells and consist of immunoglobulin molecules that form type I transmembrane receptor proteins. The heavy chain BCR includes IgA, IgD, IgE, IgM, and IgG, and consists of four gene fragments (VDJC) in the variable region (V), variable region (D), joining region (J), and the constant region (C); the light chain BCR includes IgK and IgL, lacking variable region D and consists of three gene fragments (JVC). Through high-throughput sequencing, the base sequences and sequence abundance of these BCR genes can be comprehensively resolved and finally analyzed to obtain the immune characteristics of the organism.

Customers Need to Provide

Samples

Customers only need to inform the BCR strand to be measured and provide samples of B cells, whole blood, bone marrow, or tissue types as requested. We will complete a series of work such as sample RNA extraction, receptor library creation, sequencing, and bioinformatics analysis.

• For blood samples, the sample volume should be no less than 0.5 mL.
• For fresh/frozen tissue samples, the sample weight should be no less than 500 mg.
• For peripheral blood mononuclear cell samples, the sample cell count should be greater than 2 × 10⁵.

Please note that all samples should be stored at -80°C. Do not freeze and thaw repeatedly during sample storage. Please ship samples with a sufficient amount of dry ice when sending samples.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Antibody Screening.
• Prediction and intervention guidance for transplant rejection.
• Screening of human primary immunodeficiency disease cells for mutations.
• Guidance for the treatment of autoimmune diseases and the monitoring of their course.
• Assessment of the efficacy and tolerance of drug therapy for infectious diseases or malignancies.

Our Advantages

• Our BCR sequencing service covers different species.
• Strict quality control process to reduce the false-positive rate and ensure true sequencing data.
• With rich sequencing experience, we can solve the problems encountered in sequencing analysis for you in a targeted manner.

Our Workflow

CD Genomics is a biotech company with a professional sequencing platform and a long-standing focus on B-cell receptor sequencing service and can provide a personalized B-cell receptor sequencing solution based on the actual situation of your experiment. If you want to achieve rapid and cost-effective B-cell receptor analysis in your research, please feel free to contact us for technical support.

For research use only, not for any clinical use.