De Novo Genome Sequencing

De Novo Genome Sequencing

De novo genome sequencing can sequence the genome of a species without a reference genome, mapping the genome sequence of that species and providing a database for subsequent studies. CD Genomics has long been committed to sequencing services, providing researchers with de novo genome sequencing service based on long read sequencing technology to help them conduct more in-depth studies of the whole genome.

What Is De Novo Genome Sequencing?

De novo genome sequencing refers to sequencing the genome of a species (unknown organism) without relying on any reference sequence, then splicing and assembling the obtained sequence information to map the whole genome sequence of the species and construct the genome database of the species. This helps researchers to predict gene functions and analyze the phylogeny of species. With the whole genome sequence map, it can also provide a platform for subsequent gene prediction and gene function verification, and promote the research progress of the species at the genomic level.

Customers Need to Provide

Samples

• For DNA samples, the concentration should be greater than 200 ng/μL and the total amount of DNA should be greater than 500 μg.
• For plant samples, the sample is recommended to be yellowing seedlings or histoculture samples cultured under dark aseptic conditions, and the sample should be greater than 0.5 g.
• For animal samples, samples are recommended for tissues with low-fat content such as muscle and blood, and the sample should be greater than 0.5 g.

Please note that sampling should be performed from the same individual to reduce the effect of individual differences on splicing. Keep the sampling process as cold as possible. All samples should be stored at -80°C. Samples should not be repeatedly frozen or thawed and transported on an adequate amount of dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Comparative genomics studies.
• Evolutionary analysis of plants and animals.
• Gene prediction, gene function annotation.

Our Advantages

• Based on the PacBio platform, it has the characteristics of long-read sequencing and high precision.
• Our de novo genome sequencing service can target plants and animals, bacteria, fungi, and more.
• We have rich sequencing experience and can specifically solve various problems encountered in de novo genome sequencing for you.

Our Workflow

As a biotechnology company with a professional sequencing platform, CD Genomics has rich experience in de novo genome sequencing and can provide a one-stop de novo genome sequencing service. If you are engaged in related research, please feel free to contact us for professional technical support.

For research use only, not for any clinical use.