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Long Read DNA Methylation Sequencing
DNA methylation can alter chromatin structure, DNA conformation, DNA stability, and the way DNA interacts with proteins without altering the DNA sequence, thus changing the genetic expression of organisms. As an important epigenetic mechanism, DNA methylation is involved in a variety of important life activities of organisms and plays an important role in life processes. CD Genomics, one of the most advanced sequencing companies, provides researchers with a DNA methylation sequencing service based on long read sequencing platforms Oxford Nanopore and PacBio SMRT.
What Is Long Read DNA Methylation Sequencing?
Bisulfite conversion sequencing and immunoprecipitation sequencing are commonly used to detect DNA methylation, but the conversion efficiency of bisulfite conversion sequencing is limited and short-read sequencing cannot accurately identify repetitive regions of the genome; immunoprecipitation sequencing cannot achieve single-base resolution. DNA methylation sequencing based on Oxford Nanopore and PacBio SMRT can overcome these drawbacks.
DNA Methylation Sequencing Based on Oxford Nanopore
Oxford Nanopore conducts sequencing by inferring the base composition based on the current change of single-molecule DNA passing through the biological nanopore. The four bases of ATCG and the bases with methylation modification have different charged properties, so the type of bases passing through the nanopore can be detected by the difference in the electrical signal, so as to realize the detection of DNA methylation modification.
DNA Methylation Sequencing Based on PacBio SMRT
In PacBio SMRT, DNA polymerase catalyzes the incorporation of fluorescently labeled nucleotides into complementary nucleic acid strands, enabling the direct detection of modified nucleotides in DNA templates, including N6-methyladenine, 5-methylcytosine, and 5 -Hydroxymethylcytosine. Different modifications have different effects on polymerase kinetics, and the methylation modifications of DNA molecules were detected by the difference in the interpulse duration ratio (IPD ratio).
Customers Need to Provide
Samples
We accept many forms of samples including DNA samples, cell samples, tissue samples, and more.
Please note that the sample extraction process should be kept at a low temperature as possible, and please confirm the purity of DNA samples. All samples should be stored at -80°C. Samples should not be frozen and thawed repeatedly. Use sufficient dry ice for transport.
Sample Information
Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).
Deliverables
- Sequencing raw data
- Data analysis report
- Experimental results type files
- Graphs required for publication
- Descriptions of some of the methods used in the published article
Applications
- Detection of genetic diseases and oncogenes.
- Explore the role of methylation modifications in embryonic development.
- Explore the link between methylation modifications and regulation of gene expression.
Our Advantages
- Direct detection of DNA methylation modifications without bisulfite treatment.
- Detect methylation modifications of DNA at the single-base resolution level.
- It has been widely used in complex animal and plant genomes, microbial genomes, and human genomes.
- Long-read sequencing enables the detection of repetitive regions of the genome and mapping of methylation patterns in highly repetitive genomic regions.
Our Workflow
CD Genomics is committed to meeting all of our customers' sequencing needs. We are now able to offer our customers DNA methylation sequencing services based on the Oxford Nanopore and PacBio SMRT. If you are conducting a DNA methylation study, please feel free to contact us for technical support.
For research use only, not for any clinical use.
