Hi-C Seq

Hi-C Seq

At CD Genomics, we utilize our cutting-edge sequencing platform and professional technical team to provide Hi-C sequencing services to researchers to help them capture spatial interaction information between different gene seats on a genome-wide scale and study the DNA components that regulate genes in 3D space.

What Is Hi-C Seq?

Hi-C technology is derived from genome capture technology. This sequencing method uses high-throughput sequencing technology to analyze the three-dimensional spatial structure of chromatin using the entire cell nucleus as the target for studying three-dimensional genomes.

Customers Need to Provide

Samples

• For cell samples, the total amount should be more than 2.5 × 10⁶, and it is recommended to reach 5 × 10⁷ to 1 × 10⁸ to ensure the reproducibility of the experiment.
• For tissue samples, a minimum of 100 mg of animal tissue is required and 200-300 mg is recommended to ensure experimental reproducibility.
• For DNA samples, the total amount is 15-20 µg.

Please note that cell and tissue samples need to be cross-linked and fixed with formaldehyde. All samples should be transported on an adequate amount of dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article
• High-resolution mapping of chromatin regulatory element interactions

Applications

• Chromatin structure analysis.
• Gene expression regulation.
• Structural variation analysis.
• Study of disease mechanisms.
• Dissecting genome-wide interaction patterns.
• Construction of genome-wide interaction maps.
• Assisting in genome assembly enhancement.
• Constructing genome haplotype maps.
• Quantifying interchromatin crosslinks in the genome in three dimensions.

Our Advantages

• We have professional technicians with proven library construction techniques.
• No need to build a specialized population, or single sample to achieve assisted genome assembly, sequencing, and targeting.
• Can be combined with ATAC-seq, ChIP-seq, and other technologies to customize the content of your analysis.
• Our Hi-C seq covers human, mouse, rat, avian, mammalian, fish, shellfish, microbial, plant, and many other species.

Our Workflow

CD Genomics provides researchers with Hi-C seq for analyzing the three-dimensional spatial structure of chromatin using high-throughput sequencing technology combined with bioinformatics analysis methods. if you have a need, you can contact us directly for technical support.

For research use only, not for any clinical use.