Single-Nuclei RNA Sequencing

Single-Nuclei RNA Sequencing

Single-nuclei RNA sequencing (snRNA-seq) and single-cell RNA sequencing are both important methods for studying cellular gene expression, the difference lies in their applicability to different sample types. CD Genomics, as a biotechnology company focused on sequencing, provides researchers with single-nuclear RNA sequencing services based on our high-end sequencing technology platform to help their in-depth study of gene expression.

What Is Single-Nuclei RNA Sequencing?

Single nuclei RNA sequencing is an alternative to scRNA-seq technology, which analyzes the nucleus of a cell rather than the intact cell. scRNA-seq requires too high quality for sequencing samples, but single-nuclear RNA sequencing solves this challenge well with good sample suitability. snRNA-seq mainly determines nuclear transcriptome, so it is more suitable for those cells that are difficult to isolate, such as adipocytes, neurons, etc. Moreover, snRNA-seq can reduce the generation of pseudocellular taxa induced by enzymatic digestion and mechanical stress, and obtain rich genetic data such as intronic regions and intergenic regions.

Customers Need to Provide

Samples

• For tissue samples, fresh, frozen, or lightly fixed tissue is required, with a sample volume of not less than 1 g.
• For cell samples, cell activity and morphological integrity are required, with a sample volume of not less than 1 µL.

Please note that the sampling or extraction process should be kept as cold as possible. All samples should be stored at -80°C, not repeatedly frozen and thawed, and transported on an adequate amount of dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Identification of cellular taxa.
• Uncovering gene regulation mechanisms.
• Exploring cellular components and cellular characteristics.

Our Advantages

• High standard quality control steps to ensure the authenticity of sequencing data.
• Professional single-nuclear sequencing data processing methods and personalized analysis methods and processes.
• Professional and complete single-cell sequencing service platform with personalized and customized experimental protocols.

Our Workflow

CD Genomics has a long-term focus on single-nuclear RNA sequencing technology services, and we provide you with scientifically feasible experimental solutions. If you are conducting research related to cellular gene expression levels, please feel free to contact us for technical support.

For research use only, not for any clinical use.