Single Cell Sequencing

Single Cell Sequencing

Single-cell sequencing is an emerging technology in biology. CD Genomics has a robust single-cell sequencing platform, and we use the 10x Genomics Chromium system to segment single cells and generate a high-resolution, complete view of the single-cell genome using next-generation sequencing technology or PacBio SMRT.

Single Cell Sequencing Services

Single-cell sequencing relies on NGS technology to perform high-throughput sequencing analysis of the genome and transcriptome at the individual cell level, which can detect the genome or transcriptome of a single cell, reveal heterogeneous differences between cells, and provide a high-resolution map of intercellular variation. Single-cell sequencing addresses the shortcomings of traditional bulk sequencing technologies that analyze large samples of thousands or even millions of cells, reflecting the overall average results of the cell population. The specificity of genetic information expression profiles at the single-cell level plays an important role in fields such as oncology, developmental biology, and neuroscience. To help researchers explore genetic information at the cellular level, we offer the following sequencing services to our clients.

Single-Cell RNA Sequencing (scRNA-Seq): Helps researchers study cellular function and better understand intercellular differentiation, cell lineage relationships, and disease evolution by studying gene expression within a single cell.

Single-Cell ATAC Sequencing (scATAC-Seq): Identifies open regions of chromatin at single-cell resolution. When examining heterogeneous cell populations, changes in chromatin accessibility between cell subpopulations in a single sample can be identified.

Single-Nuclei RNA Sequencing (snRNA-Seq): mainly determines the nuclear transcriptome, which is more suitable for those cells that are difficult to isolate, and snRNA-seq can reduce the generation of pseudocellular taxa and obtain rich genetic data such as intronic regions and intergenic regions.

Our Sequencing Platforms

• HiSeq X Ten
• NovaSeq 6000 NGS System
• 10× Genomics Chromium System, etc.

Applications

• Embryo developmental studies.
• Preimplantation genetic diagnosis (PGD).
• Study of germ cell genetic recombination patterns.
• Exploration of small-scale cellular variation in cancer.
• Identify different cell types with their expressed genes.
• Exploring the pathogenesis of autoimmune diseases and exploring new therapeutic strategies.

Our Workflow

CD Genomics, we offer scRNA-seq, scATAC-seq, and snRNA-seq services to help our clients be able to reveal the gene sequences and expression modifications of individual cells, reflecting the heterogeneity between cells. We can provide to meet any needs of our clients, and you can contact us with any questions you may have.

For research use only, not for any clinical use.