Micro-C Seq

Micro-C Seq

CD Genomics has been focused on providing our clients with high-quality sequencing services that meet all their research needs. We provide researchers with cutting-edge micro-C sequencing service based on our excellent team and professional sequencing technology platform to help reveal finer 3D structures in cells (chromatin loops, hairpin structures, etc.).

What Is Micro-C Seq?

Micro-C seq is a 3D genomics sequencing technology developed from Hi-C seq. In this technique, micrococcal nuclease (MNase) is used instead of the restriction endonuclease in the traditional Hi-C technique to excise genomic regions that do not bind to nucleosomes, fragment chromatin into small fragments of single nucleosome length (about 146 bp), and the analyzable region covers the entire genome uniformly, making chromosome interaction mapping resolution to the nucleosome level.

Customers Need to Provide

Samples

• For cell samples, the total amount should be more than 2.5 × 10⁶, and it is recommended to reach 5 × 10⁷ to 1 × 10⁸ to ensure the reproducibility of the experiment.
• For tissue samples, a minimum of 100 mg of animal tissue is required and 200-300 mg is recommended to ensure experimental reproducibility.
• For DNA samples, the total amount is 15-20 µg.

Please note that cell and tissue samples require formaldehyde cross-linking for fixation. All samples should be stored at -80°C and transported on sufficient dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Chromatin structure analysis.
• Structural variation detection.
• Analysis of enhancer and promoter interactions.
• Analysis of chromatin interactions and chromatin topology.

Our Advantages

• Uniform coverage of the genome.
• Provides the highest resolution chromatin conformation data.
• Using MNase, chromatin fragmentation is not affected by restriction endonucleases.
• Improves the signal-to-noise ratio of experimental data to detect interactions between nucleosomes.
• The improved signal-to-noise ratio of long-range interaction and nucleosome protection sequence data.

Our Workflow

CD Genomics, a sequencing company representing international standards, offers customers a micro-C sequencing service capable of displaying chromatin conformation at the nucleosome level of resolution. If you are interested in our micro-C seq, please contact us directly.

For research use only, not for any clinical use.