Whole Genome Sequencing

Whole Genome Sequencing

Whole genome sequencing is an important technology for studying diseases and evolutionary variation in species. CD Genomics is a biotechnology company with a high-end sequencing technology platform and research team that can provide a whole genome sequencing (WGS) service to a wide range of research scholars and teams for their related research.

What Is Whole Genome Sequencing?

Whole genome sequencing is the sequencing of an organism's entire genome at once as a way to determine the genome sequence of previously unsequenced species to study biological evolution or species differences. It can also be used to recognize the root cause of disease and to prevent it or treat it correctly and early. Almost any type of cell can be a source of DNA for WGS. Whole genome sequencing can detect a large amount of variation information including single nucleotide polymorphism (SNP), insertion deletion (InDel), structural variation (SV), and copy number variation (CNV) through sequence alignment.

Customers Need to Provide

Samples

The amount of DNA we need will depend on genome size, DNA fragment length, and the required depth of coverage.

• Typically, we require 1.5 µg of DNA packed in 1.5-1.7 mL microcentrifuge tubes or 2 mL screw-cap tubes.
• For libraries that cannot be amplified, we need much larger amounts of DNA. For mammals, we need 40-80 µg of high molecular weight DNA (25 kb or larger). For microbes, we need 5-7 µg of high molecular weight DNA (10 kb or larger). For 10× Genomics libraries, we need 100 ng of DNA and the average size of the DNA should be at least 50 kb.

Please note that to ensure that each sample is sufficiently pure, we strongly recommend that all DNA be processed by phenol: chloroform extraction before submission. All samples should be stored at -80°C and shipped on sufficient dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Library preparation

Depending on the goals of the project and the size of the genome, we offer different WGS strategies. This can be very helpful in assigning repetitive regions to appropriate locations in the genome, providing a more complete assembly or alignment.

• Alignment with references: PCR-free small insert library sequenced on the Illumina platform.
• De no assembly: large insert libraries sequenced on Pacific Biosciences Sequel with small insert libraries sequenced on the Illumina platform for error correction.
• Alignment with references or de no assembly: The 10× Genomics library provides linked reads spanning 50-150 kb fragments.

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Tumor research.
• Pathogenesis of genetic diseases.
• Disease development and risk screening.
• Drug trials and pharmacogenomics.
• Species identity, population evolution of plants and animals.

Our Advantages

• Extensive experience in sample handling (including degradable samples).
• Extensive experience in sequencing projects and the ability to guarantee data quality in the context of quality control.
• Provide personalized experimental protocols with targeted solutions to the problems you encounter.

Our Workflow

CD Genomics has a long-standing focus on whole genome sequencing service. We provide whole genome sequencing service in various fields such as genetic diseases, complex diseases, genetic breeding, etc., and can help you to complete your related research. If you are interested in our projects, please feel free to contact us for technical support.

For research use only, not for any clinical use.