Next-Generation RNA-Seq

Next-Generation RNA-Seq

RNA-seq is a powerful next-generation sequencing method capable of detecting changing transcriptomes. CD Genomics uses high-throughput and accurate RNA-seq to reveal gene expression profiles and describe continuous changes in the transcriptome. We sequence cDNA libraries using either short-read or long-read strategies using state-of-the-art sequencing platforms. With extensive experience and strong sequencing capabilities, CD Genomics offers comprehensive RNA sequencing services to meet a wide range of research goals.

Next-Generation RNA-Seq Services

Our next-generation RNA-seq service includes all eukaryotes and prokaryotes, animals, plants, microorganisms, etc. in our range of detection species. With a 5-log dynamic range, RNA-seq provides excellent relative transcript quantification and enables the detection of rare transcripts. Multiple RNA types can be sequenced, including polyadenylated [polyA(+)] and non-polyadenylated mRNA, non-coding RNA, and microRNA, among others.

• Total RNA-Seq: polyA+/rRNA-RNA sequencing for analysis of differentially expressed genes or transcriptome assembly. Sequencing of the 3'-end of polyA+ RNA for analysis of differentially expressed genes or identification of transcriptional termination sites (TTS).
• mRNA-Seq: Accurate and highly sensitive quantification of gene expression, identification of known and novel transcript isoforms, gene fusions, and other features.
• miRNA & Small RNA-Seq: miRNA & small RNA sequencing for expression profiling. Provides small RNA discovery and analysis.
• LncRNA-Seq: Sequencing studies of large RNA such as long-stranded non-coding RNA to obtain fast, comprehensive, and accurate information on all large RNA transcript data related to specific biological processes (e.g., development, disease, etc.).
• CircRNA-Seq: Analysis of circRNA species in samples and expression quantification, combined with bioinformatics mining tools to study the function and regulatory mechanism of circRNA.
• CAGE-Seq: CAGE-seq combines mRNA capping site identification and high-throughput sequencing to obtain accurate TSS and 5'UTR information on a genome-wide scale.

Our Sequencing Platforms

• NextSeq 500 NGS System
• Two MiSeq NGS Systems
• NovaSeq 6000 NGS System

What Customers Need to Consider?

• Do you need single-end or paired-end reads?
• Which reads length do you need?
• How many reads per sample do you need? Recommendations range from 15mio (low resolution broad transcriptional profiling), to 25‐40mio (standard transcriptional profiling), and more if SNPs are investigated.
• In case the number of samples dictates to perform several sequencing runs, how would you like your samples to be split across runs to minimize batch effect?

Applications

• Identify variable splice sites.
• Probing allele-specific expression.
• Discovery of novel transcripts and isoforms.
• Monitor changes in gene expression over time or environment.

Our Workflow

CD Genomics provides high-quality next-generation RNA sequencing services to researchers. We are able to perform an in-depth bioinformatics analysis of all RNA transcripts to help you explore the underlying transcriptional and regulatory network mechanisms. If you are interested in this, please feel free to contact us.

For research use only, not for any clinical use.