Methyl-Seq

Methyl-Seq

DNA methylation sequencing is one of the main methods for epigenetic-related research. With rich sequencing experience and a professional technical team, CD Genomics provides methyl-seq services for researchers engaged in epigenetic-related research to map genome-wide DNA methylation at single-base resolution.

Methyl-Seq Services

DNA methylation is important epigenetic marker information, and obtaining genome-wide methylation level data for all C loci is important for epigenetic spatiotemporal specificity studies. With the development of next-generation sequencing (NGS) technology, Methyl-seq can detect the methylation patterns of CpG, CHH, and CHG regions from the whole genome level.

• Whole Genome Bisulfite Sequencing: This method converts unmethylated C bases in the genome to U by bisulfite treatment while retaining all methylated C bases. The methylation sites in the genome are detected by high-throughput sequencing technology to map the whole-genome DNA methylation at single-base resolution.
• Reduced Representation Bisulfite Sequencing: This method uses MspI digestion of the sample before bisulfite processing to obtain enrichment information for high CpG regions, reducing the number of nucleotides required for sequencing to 1% of the genome, enriching as many DNA fragments containing CpG sites as possible with a smaller amount of data, thus discovering methylation at the individual nucleotide level.

Our Sequencing Platforms

• NextSeq 500 NGS System
• NextSeq 2000 NGS System
• NovaSeq 6000 NGS System, etc.

Applications

• Probe the methylation of almost every cytosine in the genomes of most species.
• Investigate non-coding RNA-mediated DNA methylation modifications and their functions.
• Explore the methylation patterns of CpG, CHH, and CHG regions in the human genome.
• Explore the specific role of methylation modifications in biological processes such as cell differentiation, transcriptional regulation, and chromatin remodeling.

Our Workflow

CD Genomics, a representative company of next-generation sequencing, provides powerful solutions for methylation sequencing. If you are in need of this service, please feel free to contact us.

For research use only, not for any clinical use.