Library Preparation

Library Preparation

Library preparation is the first step in next-generation sequencing. Before a DNA or RNA sample can be sequenced, the nucleic acid must be isolated, fragmented, end-repaired, and covalently ligated to a junction using ligation or labeling methods. CD Genomics has a skilled library construction team that can make a wide range of libraries compatible with all of our sequencing platforms. You provide high-quality DNA or RNA and we build the libraries. We prefer to start the sequencing project at this point, which allows our experienced hands to control each step for optimal performance and ensures the highest success rate in the end. The main types of library preparation services we offer are listed below.

Our Library Preparation Services

DNA Libraries Preparation

The genome is the sum of all the genetic material of an organism, with consistency, stability and continuity, and the study of the genome is of great importance for understanding the evolution of life.

• DNA fragment libraries
• Metagenomic libraries
• Bacterial 16S ribosomal
• Fungal ITS
• Genotyping-by-Sequencing (ddRAD)

RNA Libraries Preparation

Transcriptome refers to the entire transcriptional products in a specific cell or tissue, including mRNA, rRNA, tRNA, and other non-coding noncoding RNAs (nc RNA). Transcriptomics further advances the study of gene function and nc RNA regulatory mechanisms by studying the expression levels of gene coding RNAs and non-coding RNAs under specific conditions and their regulatory laws.

• RNA-Seq (transcriptome or Poly-A-selected mRNA-Seq)
• Small RNA-Seq (microRNAs and other small, non-coding RNAs)
• Ribo-depleted libraries (We ask for at least 2 ug of total RNA for the preparation ribo-depleted libraries.)
• 3'-Tag-Seq (For high-throughput 3'Tag-Seq library generation we require pure total RNA samples at a concentration of 100 ng/µL. For custom 3'-Tag-Seq library preps the input amounts can be a low as 10 ng total.)

High-Throughput (HT) Library Preparation

We offer high-throughput sequencing library prep (reduced HT rates starting from 24, 48, and 96 samples) for genomic DNA library prep as well as RNA-seq library prep after poly-A enrichment or ribose depletion.

• DNA or RNA; starting from 24 libraries

Methyl-seq Libraries Preparation

• WGBS (Whole Genome Bisulfite Seq)
• RRBS (Reduced Representation Bisulfite Seq)

ChIP-seq Library Preparation

We offer library preparation services constructed from chromatin immunoprecipitation material. For these complex experiments, we recommend that you discuss the suitability of starting materials and construction strategies with our staff. We require the submission of de-crosslinked and sonicated DNA samples for ChIP-seq. Fragments should ideally be between 100 and 300 bp in length. This will produce the tightest peaks for your data. It is recommended to keep fragment lengths under 500 bp in all cases.

Reduced Representation Library Preparation

Custom Libraries Preparation

We have experience with a diverse range of library applications and can assist with the development, troubleshooting and standardization of specific procedures. Examples include:

• Ultra-low input DNA or RNA libraries
• Tag-Seq (3' poly-A-directed mRNA-seq)
• 2b-RAD
• Mate-pair (long-insert) libraries

Our Workflow

CD Genomics' professional molecular biologists have extensive experience in DNA or RNA library creation. Our standard operating procedures (SOPs) are carefully designed, and adapters and primers for multiplexing allow us to barcode many different samples so they can be run together. If this is of interest to you, please feel free to contact us.

For research use only, not for any clinical use.