TCR Beta Sequencing

TCR Beta Sequencing

With the development of precision medicine in oncology, there is an increasing interest in the study of patients' autoimmune systems. TCR sequencing enables the analysis of the diversity and clonality of TCR libraries by detecting bulk or single cells, which is essential for attacking tumor therapies. CD Genomics provides researchers with a TCR beta sequencing service based on next-generation sequencing platforms.

What Is TCR Beta Sequencing?

TCR is located on the surface of T cells and consists of a majority of αβ TCR, and a minority of γδ TCR, which is highly diverse heterodimers. Each TCR chain consists of an antigen-recognized variable region and a constant region with three complementary decision regions (CDR). TCR α and γ chains are generated by VJ recombination, and β and δ chains undergo VDJ recombination, a rearrangement mechanism that confers diversity to the TCR library. Therefore, sequencing and analysis of the TCR library are important for enhancing the understanding of the immune system. TCR beta sequencing focuses attention on all regions of the TCR beta chain including CDR1, CDR2, and CDR3 to facilitate immuno-oncology studies.

Customers Need to Provide

Samples

• Blood
• Sorting Cells
• Fresh Frozen Tissue

Please note that all samples should be stored at -80°C. Do not freeze and thaw repeatedly during sample storage. Please ship samples with a sufficient amount of dry ice when sending samples.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Identify immune response biomarkers by sequencing TCR libraries and identifying antigen-specific T cells.
• Optimize the function and production of potentially therapeutic T cells by identifying TCR β chain sequences.
• To investigate markers of immune-mediated adverse reactions (IMAEs) by analyzing allele-specific polymorphisms in the CDR1, CDR2, and CDR3 regions that may be associated with autoimmune diseases.

Our Advantages

• Sample requirements can be as low as 10 ng - 1 μg.
• Our service process is open and transparent to ensure data security.
• Our services allow you to decipher the cancer-immune cycle and advance precision immunotherapy.
• Our NGS-based sequencing service provides you with highly accurate TCR β chain gene rearrangement data.

Our Workflow

CD Genomics is an experienced expert in sequencing with an advanced sequencing technology platform and extensive sequencing services. Our NGS-based TCR beta sequencing service can help researchers make significant progress in their immuno-oncology studies. If you happen to need this service, please feel free to contact us.

For research use only, not for any clinical use.