Whole Exome Sequencing

Whole Exome Sequencing

CD Genomics, a sequencing-focused biotechnology company, provides a whole exome sequencing (WES) service to researchers based on our high-end sequencing technology platform to help them delve into the study of genomic variation.

What Is Whole Exome Sequencing?

Whole exome sequencing is the high-throughput sequencing of whole genome exome regions using sequence capture technology to capture and enrich DNA, and is commonly used to identify rare or common variants associated with disease or phenotype in humans. Although the entire human exome represents only 1-2% of the human genome, the protein-coding region contains 85% of disease-causing mutations, and WES sequencing is deeper and more efficient than WGS, and can significantly reduce cost and time. WES is able to assist researchers in a wide range of research, including genetic disease variants, complex diseases, and cancer, through in-depth sequencing and analysis methods.

Customers Need to Provide

Samples

• For DNA samples, samples should be greater than 400 ng and A260/280 = 1.8-2.0.
• For cfDNA/ctDNA samples, samples should be greater than 50 ng, fragment size should be a multiple of 170 bp and free of genetic contamination.
• For genomic DNA samples from FFPE tissues, samples should be greater than 800 ng, and fragment sizes should be greater than 1000 bp. Please note that since DNA in FFPE is always damaged to some extent by the harsh chemical treatments used to preserve the tissue, there may be poorer sequencing data obtained.

To ensure that each sample is homogeneous and pure, we recommend that all DNA be subjected to phenol:chloroform extraction before submission. All samples should be stored at -80°C and shipped on sufficient dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Detection of genomic variants.
• Used as a tool for cancer biopsy.
• To study pathogenic mechanisms and molecular features.

Our Advantages

• We can provide a high standard and scientific experimental protocols.
• We provide professional and comprehensive bioinformatics analysis services.
• We have strict control over DNA quality, etc., to provide you with high-quality sequencing data.

Our Workflow

CD Genomics has a long-standing focus on whole exome sequencing, and we offer professional sequencing services. If you are conducting research related to genetic disease-associated variants, complex diseases, cancer research, or population genetics, feel free to contact us for technical support.

For research use only, not for any clinical use.