Targeted Sequencing

Targeted Sequencing

Targeted sequencing (TS) is commonly used for the targeted detection of variants in regions of interest and is a sequencing method that requires fewer samples, is less expensive, and produces smaller and more manageable data sets. CD Genomics offers researchers a scalable, fast, and high-resolution targeted sequencing service based on a next-generation sequencing platform that can help them evaluate multiple genes in parallel across multiple samples.

What Is Targeted Sequencing?

Targeted sequencing is a research strategy that amplifies or captures the genomic region of interest and then sequences it after enrichment. It can be used to obtain ultra-high sensitivity and accuracy with a small amount of data, enabling rapid screening of mutant loci. Targeted sequencing based on NGS technology uses deep sequencing to detect known and novel variants in regions of interest and is capable of investigating the mutational status of multiple genomic regions of interest simultaneously. In contrast to WGS and WES, the focus of TS is on probing a panel of genes or targets known to be strongly associated with disease pathogenesis and/or clinical relevance.

Targeted Sequencing Service

• Gene-Specific Sequencing
• Targeted Resequencing
• Targeted DNA Sequencing
• Targeted Exome Sequencing
• GC-Rich Region Targeted Sequencing, etc.

Customers Need to Provide

Samples

• For DNA samples, the sample volume should be ≥ 10 ng and the DNA concentration should be ≥ 1 ng/μL, A260/280 = 1.8-2.0.

Please note that all DNA samples are subject to purity and concentration verification. Also, all samples should be shipped on sufficient dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Association of mutations with disease expression.
• Agricultural genomics/molecular breeding.
• Detection of SNPs/InDels/CNVs/SVs in the genome.
• Study of pathogenesis and diagnostic targets of various diseases including cancer.

Our Advantages

• High-depth sequencing identifies rare variants.
• Produces smaller, more manageable data sets, making data analysis easier.
• Can evaluate multiple genes in multiple samples simultaneously, saving testing time and cost.

Our Workflow

At CD Genomics, we offer a one-stop for targeted sequencing. From sample preparation to bioinformatic analysis of the data, we can provide satisfactory service. If you need to perform targeted sequencing of the genome, please feel free to contact us for technical support.

For research use only, not for any clinical use.