CAGE-Seq

CAGE-Seq

CAGE-seq (cap analysis gene expression and deep sequencing) is an efficient method for analyzing transcription start sites (TSS). CD Genomics, a comprehensive sequencing biology company, can provide researchers with a CAGE-seq service based on next-generation sequencing platforms. CAGE-seq can help researchers accurately identify TSS and corresponding promoter regions to annotate genomes with new perspectives.

What Is CAGE-Seq?

Transcriptional regulation is the most important way of regulating gene expression, and the core promoter located upstream of the transcription start site (TSS) is the main element of transcriptional regulation. Therefore, CAGE-seq is very important for the study of gene expression regulation. CAGE-seq is a means of combining mRNA capping site identification and high-throughput sequencing. The cap structure of mRNA exists at its 5' end, RNA is reverse transcribed using random primers, the cap at the 3' end of cDNA is biotinylated, while RNA without a cap, such as rRNA, truncated RNA, and incomplete reverse transcription RNA is digested. The cDNA is then subjected to high-throughput sequencing to obtain accurate TSS and 5'UTR information on a genome-wide scale.

Customers Need to Provide

Samples

• Our range of samples covers mammals, other vertebrates, invertebrates, plants, bacteria, viruses, etc.
• You will need to provide 3 μg of total RNA/sample for CAGE library preparation.

Please note that all samples should be stored at -80℃ and shipped on an adequate amount of dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Exploration of transcription factor binding motifs.
• Study of transcriptome heterogeneity and complexity.
• Discovery of biomarkers and/or bidirectional enhancer RNAs.
• Sequencing of transcriptional start sites and corresponding transcriptional regions contributes to the study of gene expression regulation.

Our Advantages

• With just a few samples, you can get a lot of data.
• Our professional technicians can ensure the correctness of every step of the sequencing process.
• Our advanced CAGE-seq technology can obtain accurate and true promoter information and 5'UTR information.

Our Workflow

At CD Genomics, we offer a CAGE sequencing service based on next-generation sequencing technology for researchers who need precise sequencing of the transcription start site (TSS) and the corresponding promoter region. If you need this service, please contact us directly for more detailed information.

For research use only, not for any clinical use.