Full-Length Isoform Sequencing

Full-Length Isoform Sequencing

CD Genomics is a biotechnology company specializing in long read sequencing. Based on our professional sequencing technology platform, we can provide full-length isoform sequencing (Iso-seq) service to researchers to help them deepen their research on transcriptomics challenges.

What Is Full-Length Isoform Sequencing?

Iso-seq based on PacBio's Single Molecule, Real-Time (SMRT) technology is capable of sequencing contiguous full-length transcripts containing 5'UTR to 3'poly-A tails. This method takes advantage of the long-read sequences of triple sequencing to directly sequence full-length cDNAs of reverse transcripts without interrupting the transcript, resulting in full-length transcript isoforms. Iso-seq is widely used in medical and agricultural neighborhoods as a high-throughput method for annotating genomes, discovering novel transcripts, probing gene mechanisms, and characterizing isoform diversity.

Customers Need to Provide

Samples

• For total RNA samples, the sample content should be greater than 800 ng, the sample concentration should be greater than 60 ng/μL, the RIN value should be greater than 6.5, A260/280 = 1.8-2.2, A260/230 = 1.3-2.5, NanoDrop concentration/Qubit concentration ≤ 2.5.

Please note that the sampling or extraction process should be kept as cold as possible. All samples should be stored at -80℃, not freeze and thaw samples repeatedly, and transported on an adequate amount of dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Better annotation of genomes.
• Study of disease mechanisms.
• Study of drug resistance mechanisms.
• Explore the discovery of new genes, transcripts, or isoforms.
• For the discovery of selectively spliced transcripts as potential biomarkers.
• To reveal plant growth and development under different environmental stresses.

Our Advantages

• Short experimental cycle time and economical price.
• Professional and scientific workflow to ensure accurate and reliable sequencing data.
• Experienced sequencing team to provide a well-developed sequencing pipeline.

Our Workflow

CD Genomics has a long-standing focus on isoform sequencing technology services, providing high-quality data and cost-effective protocols that are designed to high scientific standards at every step of the process to ensure high-quality research results. If you are conducting research related to disease mechanisms, drug resistance mechanisms, new gene discovery, etc., please feel free to contact us for technical support.

For research use only, not for any clinical use.