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Protocol Development
Sequencing workflows are developed depending on the type of study to be conducted. To carry out a successful sequencing, the experimenter must have a standardized workflow that addresses the various basic issues that arise, including sample collection, sample preparation, data collection, data processing and documentation, and data analysis. Standardization of workflows is, therefore, key to robust and reproducible sequence data production. CD Genomics can provide protocol solutions appropriate to the different needs of the research team as well as the researcher.
Our Protocol Development Service
CD Genomics has standardized chromatin workflows for a variety of primary tissues (brain, blood, muscle) and different living organisms (embryos, single-cell organisms). For different types of cells, we have introduced a method called Nuclei EXtraction by SONication (NEXSON) to address the cell preparation process. This method is capable of extracting the nuclei of our target cells from a wide range of formaldehyde fixation materials. The versatility and sensitivity of the method have been well demonstrated using a ChIP-Seq workflow to process the nuclei we need.
To enable standardized high-throughput ChIP-Seq, a method called RELACS (restriction enzyme-based in situ labeling of chromatin) was introduced. RELACS can process standardized extracted nuclei from any source and employ chromatin cleavage and encoding of intact nuclei. The bar-coded nuclei are processed centrally in the same ChIP reaction to increase efficiency. This type of approach maximizes the scale and speed of sequencing and maintains versatility, scalability, and enables automation. RELACS, for example, can generate ChIP-seq libraries of transcription factors and histone modifications from hundreds of samples in three days.
Our Workflow
CD Genomics is a leader in the sequencing industry, with a professional sequencing technology platform and technical team, providing high-quality protocol development services for various sequencing projects. If you are interested in our related services, feel free to contact us for expert support!
References
- Arrigon.; et al. Standardizing chromatin research: a simple and universal method for ChIP-seq. Nucleic Acids Research. 2016, 44(7): e67.
- Arrigoni.; et al. "RELACS nuclei barcoding enables high-throughput ChIP-seq." Communications Biology. 2018, 1: 214.
- Arrigoni.; et al. AutoRELACS: automated generation and analysis of ultra-parallel ChIP-seq. Scientific Reports. 2020, 10(1).
For research use only, not for any clinical use.
