Single-Cell ATAC Sequencing

Single-Cell ATAC Sequencing

Single-cell ATAC sequencing (scATAC-seq) technology to detect chromatin accessibility and explore cellular transcriptional regulation mechanisms is one of the important directions in studying epigenetics. CD Genomics, a biotechnology company with an advanced sequencing technology platform and R&D team, has long been dedicated to single-cell ATAC sequencing to provide researchers with quality sequencing service.

What Is Single-Cell ATAC Sequencing?

Single-cell ATAC sequencing is a new assay combining single-cell technology and traditional ATAC-seq. scATAC-seq identifies open regions of chromatin at single-cell resolution. When examining heterogeneous cell populations, changes in chromatin accessibility between cell subpopulations in a single sample can be identified. scATAC-seq solves the previously existing cellular heterogeneity challenge and expands the scope of application of the technology to further deepen the genetic regulatory mechanism of cellular heterogeneity epistasis.

Customers Need to Provide

Samples

Human and mouse genomes can be tested. (Other species require higher quality genomes.)
Sample types: cell lines, primary cells, frozen cells, fresh tissues, etc.

• For single-cell suspensions, need to be live fine and >80% cell activity, cell concentration in the range of 700 to 1200 cells/μL, the total number of cells needs to be 10⁶, and cell clumping rate less than 5%.
• For blood samples, EDTA-anticoagulated whole blood >5 mL (must not contain heparin).
• For tissue samples, fresh tissue >200 mg is required. Note that the assay is not compatible with FFPE samples as the formalin fixation process can lead to DNA degradation.

Please note that all samples should be stored at -80°C and transported on an adequate amount of dry ice.

Sample Information

Please provide the specific concentration, volume, preparation time, and species origin for each sample. And inform about the specific information as well as the control and experimental samples (if there are groupings, describe in detail the grouping analysis information).

Deliverables

• Sequencing raw data
• Data analysis report
• Experimental results type files
• Graphs required for publication
• Descriptions of some of the methods used in the published article

Applications

• Mapping chromatin open regions.
• Study of tumor epigenetic mechanisms.
• Prediction of potential markers of disease.
• Probing the developmental trajectory of embryonic cells.
• Cellular heterogeneity prediction of novel cell subpopulations.

Our Advantages

• Multiple quality control steps to ensure authentic and reliable data.
• We provide one-stop service from sample processing, scATAC library building, sequencing, and analysis.
• Our scATAC seq is suitable for a wide range of sample types such as cell lines, primary cells, frozen cells, fresh tissues, etc.

Our Workflow

CD Genomics has a long-standing focus on epigenetic sequencing services, and we offer scATAC-seq to help study chromatin openness on a genome-wide scale at the single-cell level. If you are conducting related research, feel free to contact us for technical support.

For research use only, not for any clinical use.