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TaqMan SNP Genotyping Assays Service
CD Genomics has a complete sequencing platform and a strong technical team to provide professional TaqMan SNP genotyping assays for research teams and scholars working in genomics and other related fields to meet all your experimental needs.
TaqMan SNP genotyping assays use TaqMan® 5'-nuclease to amplify and detect specific single nucleotide polymorphisms in purified genomic DNA samples. Each assay allows genotyping of individuals for single nucleotide polymorphisms (SNPs).
Assay Components
Each SNP genotyping assay mixture contains:
- Two specific primers for amplification of polymorphic sequences of interest: forward and reverse primers
- 1 VIC dye-labeled MGB probe to detect allele 1 sequences
- 1 FAM dye-labeled MGB probe for allele 2 sequences
MGB probes bind to DNA helix minor grooves at the 3' end for superior allele differentiation and assay design flexibility by stabilizing the MGB probe-template complex. The MGB probe also includes a non-fluorescence quencher (NFQ), which virtually eliminates background fluorescence and provides an excellent signal-to-noise ratio, thereby improving the sensitivity of the assay.
About the 5'Nuclease Assay
- The 5' nuclease assay process takes place during PCR amplification. It occurs in every cycle and does not interfere with the exponential accumulation of product. For the PCR amplification, genomic DNA is introduced into a reaction mixture consisting of a master mix, forward and reverse primers, and two TaqMan® probes.
- During PCR, forward and reverse primers are annealed to complementary sequences along the denatured cDNA template strand. When the probe is intact, the proximity of the reporter dye to the quencher dye results in the suppression of reporter fluorescence.
- DNA polymerase cleaves only the probes hybridized to the target sequence. The separation of the reporter dye from the quencher dye results in an increase in fluorescence of the reporter dye. The fluorescence increases only when the target sequence is complementary to the probe and amplified during the PCR. Due to these requirements, the reaction does not detect non-specific amplification while the fluorescence signal indicates which alleles are present in the sample.
The Advantages of Our TaqMan SNP Genotyping Assay Service
- Minor Groove Binder (MGB) technology enables TaqMan probes to distinguish between highly homologous allelic sequences.
- Scientifically feasible experimental design with multiple parameters for optimal primer-probe combinations.
- Functional quality testing using gDNA samples from each experiment.
Our TaqMan SNP genotyping assays use gold-standard TaqMan chemistry, scientifically feasible assay designs, and easy-to-use workflows to detect single-nucleotide differences between two alleles quickly and accurately, with reproducibility.
CD Genomics is a leader in sequencing services, and as a global company with a specialized sequencing technology platform and technical team, we value collaborative interactions to provide high-quality TaqMan SNP genotyping assays services. If you are interested in TaqMan SNP genotyping assay, please contact us today for expert support!
For research use only, not for any clinical use.
